NCT03994367NARecruitingAccepts Healthy Volunteers
Animal and Plant Proteins and Glucose Metabolism
University of Missouri-Columbia
Start Date
7/12/2019
Completion Date
4/25/2028
Summary
The goal of this proposal is to determine the effect of a high protein diet in which the increase in protein intake is derived from different sources (animal vs plant and protein-rich whole foods vs protein isolates) on: i) liver and muscle insulin sensitivity; ii) the metabolic response to a meal, and iii) 24-h plasma concentration profiles of glucose, glucoregulatory hormones, and protein-derived metabolites purported to cause metabolic dysfunction.
Eligibility Criteria
Age Range: 21 years to 70 years
Inclusion Criteria:
* age: ≥21 and ≤70 years;
* BMI: \>24.5 and \<32.5 kg/m2;
* habitual protein intake \<0.9 g/kg/day (assessed on 2 weekdays and 2 weekend days by using the HealthWatch 360 app); and
* weight stable (i.e., ≤3% change) and untrained (≤150 min of structured exercise/week) for at least 2 months before entering the study.
Exclusion Criteria:
* prediabetes or type 2 diabetes;
* evidence of chronic kidney disease by medical history or laboratory tests (glomerular filtration rate \<60 ml/min/1.73 m2 or an albumin to creatinine ratio in urine ≥30 mg/g);
* vegetarians or vegans;
* intolerance or allergies to ingredients in the metabolic meal or intervention diet;
* take dietary supplements (e.g., pre- and probiotics, fiber, fish oil) or medications known to affect our study outcomes;
* received antibiotic or antifungal treatment (which affect the microbiome and therefore microbial metabolite production) 2 months before entering the study;
* consume tobacco products or excessive alcohol (women: \>14 drinks/week; men: \>21 drinks/week);
* evidence of significant organ system dysfunction or diseases (e.g., cirrhosis), and
* unwilling or unable to provide informed consent.
Interventions
OTHER
High animal protein isolate
OTHER
High animal protein whole food
OTHER
High plant protein isolate
OTHER
High plant protein whole food
Conditions
Metabolic SyndromeMetabolic Syndrome, Protection AgainstGlucose Metabolism Disorders
Locations
University of Missouri School of Medicine
Columbia, Missouri 65212
United States